Component Table

Use this command to set up, review, or modify the Component Table for data processing. For calculation methods other than Area%/Height%, you must set up the Component Table by entering characteristics of the components that might be present in the samples. The HSM uses the information in the table to identify peaks that correspond to each component and to perform quantification and any specified functions for each component. Note that a simple Component Table appears when Area% or Height% is selected on the Calculation Method dialog. You need to set it up only if you want to perform SST or data diagnosis on one or more peaks.

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Table Entries

RT (min) Enter the expected retention time in minutes for the corresponding component. Window(%) or Window (min)The heading is determined by the selection of %Time or Absolute Time as the Peak Identification window on the Calculation Method screen. Enter a tolerance for the retention time.· If the Peak Identification window is %Time, specify the tolerance in percentage of the retention time.· If the Peak Identification window is Absolute Time, enter the absolute tolerance value in minutes.The input value is automatically converted into a percentage time (%Time) or an Absolute Time if you change the Peak Identification window from the Calculation Method screen. Name (This column is not present on the simple Component Table.) Enter the name of the component. The name can be up to 30 characters in length. Once the name is entered, it is automatically copied to the Concentration Table and the Coefficient Table. Func1, Func2, Func3 Specify functions that are used for peak identification and quantitation. The functions available for this field include the following: · Internal standard (ISTD): The ISTD function is used to designate one component as the internal standard component. This is necessary only if the Int-Std calculation method is in use. · Relative retention time reference (RRT): The RRT function is used to designate one or more components as RRT reference peaks. The HSM uses the observed RT and expected RT of an RRT component to determine Relative Retention Time for each component. See Relative Retention Time (RRT). · Corrected retention time reference (CRT): The CRT function is used to correct variations in the retention time that depend on various HPLC conditions. A maximum of 20 reference peaks can be designated by selecting CRT in Func1, Func2, or Func3 fields in the Component Table. See Corrected Retention Time (CRT). · Expected concentration peak (E-CONC): The E-CONC function is used to designate one or more components as the Concentration Peak component. Only when E-CONC is selected for a component do you need to enter the Expected concentration and the tolerance% for the component in the last two columns; otherwise, these columns are read-only. See Data Diagnosis. · System Suitability Test (SST): The SST function is used to designate one or more components peaks as SST peaks. The peaks so designated are used to generate System Suitability Test results which are reported in a Confidence Report. See System Suitability Tests (SST). · Reference component (REF) and the components to be quantified with the calibration coefficients of the reference components (USE-REF). The REF and USE-REF functions allow you to quantify components (only for Ext-Std and Int-Std calculation method) that are listed on the Component Table and are present in unknown injections but are not included in the standard injections. They are also used to quantify peaks that are not listed in the Component Table. The REF function is used to designate a component as the reference component. The USE-REF function is used to mark components in the table that are not calibrated. The calibration coefficients of the reference component are used to calculate the concentration of any component in the Component Table that is marked with the USE-REF function. Using the USE-REF function is meaningless unless one component is designated as the reference component with REF. Also, it is not valid to mark one component as the REF as well as USE-REF component or to mark the Internal Standard (ISTD) component as the REF or USE-REF component. · GROUP-1 to GROUP-20: These functions are used to group components. See Group .Note: In the simple Component Table for Area% method, only the SST and E-CONC functions are available under Func1 and Func2. · SPECTRUM: Select SPECTRUM for desired component to have its peak-top spectrum plotted in Spectra-in-Chrom graph for both display and reporting. Up to four components can be specified. A warning message is displayed if more than four are selected. See Picture-in-Picture on Chromatogram Display Format screen.. · MAIN/DEGRADE: Degradation from a main component is quantified by relative area% in the Method. (See Quantitation for Degradation.) Specify which components to use as MAIN and which to use as DEGRADE. Only one component can be defined as MAIN, but many can be defined as DEGRADE. If two components are defined as MAIN, a warning is issued. · A separate table is created to report the degradation products. A Degradation Table report item in the Report Layout Editor (in the Injection section) can be selected to display the table. Molecular Weight This column is not present in the simple Component Table for Area% method. The molecular weight is used when the concentration is calculated for the desired units in the first and second concentrations. The molecular weight have a range from 0 to 999999.000. The unit conversion for the Concentration Table uses the molecular weight. However, when concentration data from the Sample Wizard is selected, molecular weight is not used in data processing. Multiplier Specifies the multiplier for calculating Conc2 and Conc3 concentrations when the Use Component Multiplier on the Report Format screen is checked. If it isn't checked, the multiplier is not used in the calculation. E-Conc Specifies the expected concentration of the component. This field is read-only unless the E-Conc function is selected on the same row. Tolerance (%) Enter the tolerance as a percentage. This field is read-only unless the E-Conc function is selected on the same row. Import Component from Sample Wizard Library button When processing chromatogram data using Sample Wizard concentration information, the component names in the Component Table should be identical to the Sample Wizard concentration information or peaks are not identified. Use the Import Component from Sample Wizard Library button to import component name and molecular weight information directly from the Sample Wizard component library to the Component Table. This button is enabled only when all of the following conditions are true: Sample Wizard has been installed. Calculation method is not Area% or Height%. One row in Component Table is highlighted, or a component name is highlighted, or the cursor points to Name cell in the Component Table.When you click on the Import Component from Sample Wizard Library button, the Import Component Method Component Table dialog opens. Note: You can add or delete rows in the Component Table using commands in the Edit menu. When this happens, the corresponding rows in both the Concentration Table and the Coefficient Table are automatically changed accordingly.